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Vitamin B1, Plasma

Test ID: 

703153

CPT code:

84425

Synonyms:

Vitamin B1 (Thiamine), Plasma/Serum
B1,Thiamine

Clinical Use:

Vitamin B1 deficiency is most often associated with alcoholism, chronic illness and following gastric by-pass surgery. Prolonged deficiency causes beriberi. Plasma vitamin B1 is useful in evaluating nutritional assessment and compliance, while whole blood vitamin B1 is useful in evaluating body stores.

Specimen Type:

Frozen plasma – protected from light

alternate: Frozen serum in an amber transport tube or foil wrapped (light-protected)

Requested Volume: 

2 mL

Minimum Volume: 

1 mL

Container Type: 

Lavender-top (EDTA) tube

Patient Preparation: 

Overnight fasting is required

Collection:

Plasma (preferred): Collect blood in a lavender-top tube. Remove plasma within 4 hours of collection and transfer to a light protected pour off tube. If separation of cells can’t be performed immediately after collection, keep the whole blood refrigerated and protected from light. The separation of cells must be completed within 4 hours of collection. Separate cells by centrifugation at 2-8° C for 8-10 minutes. Transfer plasma to amber tube to protect from light and freeze tube immediately. Alternately, neutral colored tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30° C.
Serum (acceptable): Collect blood in plain red top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.

Storage Instructions:

Frozen

Stability Requirements:

Room temperature: 8 hours
Refrigerated: 48 hours
Frozen: 30 days

Rejection Criteria

Hemolysis • Lipemia • Received room temperature • Received refrigerated • Receive not protected from light

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