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Thrombotic Risk Assessment

Test ID: 

704568

CPT code:

81240, 83090, 85420, 85300, 85303, 85306, 85307, 85732, 85613, 86147 (x3), 86146 (x3)

Test Information:

Activated protein C resistance (APCR) reflex to factor VLeiden mutation analysis; anticardiolipin antibodies (ACA), IgA, IgG, IgM; antithrombin activity; β2-glycoprotein 1 antibodies, IgA, IgG, IgM; factor II (prothrombin) DNA analysis; homocyst(e)ine, plasma; lupus anticoagulant with reflex; plasminogen activity; protein C, functional; protein S antigen, free

Specimen Type:

Plasma, frozen, and serum, refrigerated, and whole blood or buccal swab kit (The buccal swab collection kit contains instruction for the use of a buccal swab.)

Requested Volume: 

6 mL frozen plasma (3 mL plasma into each of two tubes) and 2 mL serum and 7 mL whole blood or buccal swab

Minimum Volume: 

3 mL frozen plasma and 2 mL serum and 3 mL whole blood or buccal swabs

Container Type: 

Blue-top (sodium citrate) tube, gel-barrier tube, lavender-top (EDTA) tube, yellow-top (ACD) tube,

Patient Preparation: 

Do not draw from an arm with a heparin lock or heparinized catheter.

Collection:

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a PP transpak frozen purple tube with screw cap. The specimen should be frozen immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions:

Freeze plasma. Refrigerate serum. Maintain whole blood and buccal swab kit at room temperature or refrigerate

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