Thrombotic Risk, Acquired
85613; 85705; 85732; 86146(x2); 86147(x2)
Thrombotic Risk Profile, Acquired
Antiphospholipid Syndrome Profile
Detect lupus anticoagulants and antiphospholipid antibodies
Anticardiolipin antibodies, IgG and IgM; β2-glycoprotein 1 antibodies, IgG and IgM; dilute prothrombin time profile; lupus anticoagulant with reflex.
Serum and plasma, frozen
1 mL serum and 4 mL frozen plasma (2 mL plasma in each of two tubes)
0.5 mL serum and 2 mL frozen plasma
Red-top tube or gel-barrier tube and blue-top (sodium citrate) tubes
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, “butterfly”), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a PP transpak frozen purple tube with screw cap . The specimen should be frozen immediately and maintained frozen until tested.
Refrigerate serum. Freeze plasma.