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Thrombotic Risk, Acquired

Test ID: 

704574

CPT code:

85613; 85705; 85732; 86146(x2); 86147(x2)

Synonyms:

Thrombotic Risk Profile, Acquired
Antiphospholipid Syndrome Profile

Clinical Use:

Detect lupus anticoagulants and antiphospholipid antibodies

Test Information:

Test Includes
Anticardiolipin antibodies, IgG and IgM; β2-glycoprotein 1 antibodies, IgG and IgM; dilute prothrombin time profile; lupus anticoagulant with reflex.

Specimen Type:

Serum and plasma, frozen

Requested Volume: 

1 mL serum and 4 mL frozen plasma (2 mL plasma in each of two tubes)

Minimum Volume: 

0.5 mL serum and 2 mL frozen plasma

Container Type: 

Red-top tube or gel-barrier tube and blue-top (sodium citrate) tubes

Collection:

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, “butterfly”), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a PP transpak frozen purple tube with screw cap . The specimen should be frozen immediately and maintained frozen until tested.

Storage Instructions:

Refrigerate serum. Freeze plasma.

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