Thrombotic Risk Acquired Comp

Test ID: 

704567

CPT code:

86849 (x2), 85613, 85705, 85732, 86146 (x3), 86147 (x3), 86148 (x3)

Synonyms:

Thrombotic Risk Profile, Acquired, Comprehensive
Antiphospholipid Syndrome, Comprehensive

Clinical Use:

Detect lupus anticoagulants and antiphospholipid antibodie

Test Information:

Test Includes
Anticardiolipin antibodies, IgA, IgG, IgM, quantitative; antiphosphatidylserine IgA, IgG, IgM; β2-glycoprotein 1 antibodies, IgA, IgG, IgM; dilute prothrombin time:confirm ratio; dilute prothrombin time; dilute Russell’s viper venom time (dRVVT); LA-sensitive activated partial thromboplastin time (aPTT-LA); lupus anticoagulant reflex; lupus reflex interpretation; prothrombin antibodies, IgG

Specimen Type:

Serum and plasma (sodium citrate), frozen

Requested Volume: 

4 mL serum and 4 mL frozen sodium citrate plasma (2 mL of plasma into each of two tubes)

Minimum Volume: 

1 mL serum and 3 mL frozen sodium citrate plasma

Container Type: 

Red-top tube or gel-barrier tube and blue-top (3.2% sodium-citrate plasma) tube

Collection:

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, “butterfly”), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.

Centrifuge for 10 minutes and (using a transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a PP transpak frozen purple tube with screw cap. The specimen should be frozen immediately and maintained frozen until tested.

Storage Instructions:

Refrigerate serum. Freeze plasma.