Thrombotic Risk Acquired Comp
86849 (x2), 85613, 85705, 85732, 86146 (x3), 86147 (x3), 86148 (x3)
Thrombotic Risk Profile, Acquired, Comprehensive
Antiphospholipid Syndrome, Comprehensive
Detect lupus anticoagulants and antiphospholipid antibodie
Anticardiolipin antibodies, IgA, IgG, IgM, quantitative; antiphosphatidylserine IgA, IgG, IgM; β2-glycoprotein 1 antibodies, IgA, IgG, IgM; dilute prothrombin time:confirm ratio; dilute prothrombin time; dilute Russell’s viper venom time (dRVVT); LA-sensitive activated partial thromboplastin time (aPTT-LA); lupus anticoagulant reflex; lupus reflex interpretation; prothrombin antibodies, IgG
Serum and plasma (sodium citrate), frozen
4 mL serum and 4 mL frozen sodium citrate plasma (2 mL of plasma into each of two tubes)
1 mL serum and 3 mL frozen sodium citrate plasma
Red-top tube or gel-barrier tube and blue-top (3.2% sodium-citrate plasma) tube
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, “butterfly”), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.
Centrifuge for 10 minutes and (using a transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a PP transpak frozen purple tube with screw cap. The specimen should be frozen immediately and maintained frozen until tested.
Refrigerate serum. Freeze plasma.