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T-Lymphocyte Helper/Suppressor Profile

Test ID: 


CPT code:

86359, 86360


Helper:Suppressor Ratio
T4:T8 Analysis

Test Information:

HIV-1 infection results in a decrease of CD4 T cells, an increase in CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. Enumeration of CD4 and CD8 T cells in HIV-1 seropositive patients may be used for prognostic purposes, to monitor disease progression and retroviral therapy.

Specimen Type:

Whole blood

Requested Volume: 

Fill tube(s) to capacity.

Container Type: 

Lavender-top (EDTA) tube and yellow-top (ACD-A or ACD-B) tube


Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions:

Maintain specimen at room temperature. Do not freeze or refrigerate.

Stability Requirements:



Room temperature

2 days





Freeze/thaw cycles


Rejection Criteria

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

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