Postpartum 75-Gram Glucose Tolerance
Test ID:
703085
CPT code:
82951, 82952 (x2)
Synonyms:
•Glucose Tolerance, Gestational Diabetes (Fasting, One-hour, Two-hour, and Three-hour)
Clinical Use:
Diagnose gestational diabetes
Specimen Type:
Serum or plasma
Additional Information:
In 2013,2 the American College of Obstetricians and Gynecologists (ACOG) recommended that all pregnant women be screened for gestational diabetes mellitus (GDM)—whether by patient history, clinical risk factors, or with a 50-gram, one-hour loading test at 24 to 28 weeks of gestation to determine blood glucose levels—and suggested relying on the result of the 100-gram, three-hour oral glucose tolerance test for diagnosis (often referred to as a “two-step” method).
The American Diabetes Association (ADA)1 released standards that vary from the ACOG recommendations. The ADA standards recommend a simplified “one-step” approach to the screen and diagnosis of gestational diabetes mellitus with a 75-gram, two-hour glucose tolerance test. The test according to the ADA recommendations is Gestational Glucose Tolerance Screening and Diagnostics Test (Two-hour, ADA Recommendations)
Requested Volume:
1 mL serum or plasma each tube
Minimum Volume:
0.5 mL serum or plasma each tube
Container Type:
Gel-barrier tubes (4) or gray-top (sodium fluoride/potassium oxalate plasma) tubes (4)
Collection:
Draw a fasting blood sugar before administering 100-gram glucose, and draw blood at one, two, and three hours. The subject should remain seated and not smoke throughout the test. Submit 1 mL serum or plasma for fasting, one-hour, two-hour, and three-hour specimens. Separate serum or plasma from cells within 45 minutes of venipuncture. Gray-top tubes only, may be submitted without centrifugation. Label each tube with patient’s name and collection time interval (ie, fasting, one-hour, two-hour, and three-hour).
Storage Instructions:
Maintain specimen at room temperature.
Rejection Criteria:
Frozen gray-top tube (frozen plasma from gray-top is acceptable); stressed patient (surgery, infection, corticosteroids) should not have GTT; specimens not labeled with collection time intervals (i.e., fasting, one-hour, two-hour, and three-hour)
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