Mycoplasma pneumoniae (Respiratory) Culture
•Culture, Mycoplasma pneumoniae
Isolate and identify Mycoplasma pneumoniae
Mycoplasma pneumoniae infection is acquired via the respiratory route from small-particle aerosols or large droplets of secretions. The organism can penetrate the mucociliary barrier of respiratory epithelium and produce cellular injury and ciliostasis that may account for the prolonged cough observed clinically. Most infections are observed in older children and young adults. Early infection in infancy or childhood may increase the severity of subsequent infections. The recently described M genitalium might play a role in the pathogenesis of M pneumoniae disease.1 Cold agglutinins and Mycoplasma pneumoniae complement fixation serology have been the mainstays of diagnosis because of the limitations and long turnaround time for cultures; however, immunofluorescence techniques and immunoassays to detect antibodies to M pneumoniae are available and are the recommended diagnostic methods. These tests are not yet available for routine testing. Consult the laboratory for availability of specific tests and specific instructions for specimen collection.
Throat swabs, sputum, bronchial washings, lung tissue, tracheal aspiration.
Viral, Chlamydia, or Mycoplasma culture transport or other appropriate transport medium
Refrigerate at 2°C to 8°C
Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additives; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport conditions; specimen received after prolonged delay in transport (usually more than 72 hours); specimen stored or transported at room temperature; wooden shaft swab in transport device; unlabeled specimen or name discrepancy between specimen and request label.
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