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Mycobacterium tuberculosis Detection by NAA

Test ID: 


CPT code:

87556; 87149

Clinical Use:

Detect and identify Mycobacterium tuberculosis complex and an rpoB mutation that is associated with rifampin resistance. Per the College of American Pathologists (CAP) guidelines (MIC.64820), a culture must be performed on all samples regardless of the molecular test result. By ordering this test, the client has assumed responsibility for performance of the AFB culture.

Specimen Type:

Sputum (adult population) first morning specimen.

Requested Volume: 

2 mL of raw or expectorated sputum

Minimum Volume: 

2 mL 

Container Type: 

Sterile screw-capped vial or sterile cup; seal securely.

Patient Preparation: 

When collecting sputum, have the patient brush teeth or remove dentures and rinse mouth with water. Instruct the patient not to collect saliva.

Storage Instructions:

Refrigerate. Specimen may be stored at a maximum of 35°C for three days and then at 2°C to 8°C for seven days. Specimen may also be stored frozen (below -70°C).

Rejection Criteria:

Quantity not sufficient; inappropriate specimen source; specimen from a pediatric patient; specimen received after leaking out of transport container into the specimen bag; inappropriate or expired specimen transport device; unlabeled specimen container or container with a single patient identifier; name discrepancy between the specimen container label and the computer or the requisition; specimen received after prolonged delay (more than seven days unless frozen); specimen in a paraffin block; specimen in a fixative.

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