Mycobacterium tuberculosis Detection by NAA
Detect and identify Mycobacterium tuberculosis complex and an rpoB mutation that is associated with rifampin resistance. Per the College of American Pathologists (CAP) guidelines (MIC.64820), a culture must be performed on all samples regardless of the molecular test result. By ordering this test, the client has assumed responsibility for performance of the AFB culture.
Sputum (adult population) first morning specimen.
2 mL of raw or expectorated sputum
Sterile screw-capped vial or sterile cup; seal securely.
When collecting sputum, have the patient brush teeth or remove dentures and rinse mouth with water. Instruct the patient not to collect saliva.
Refrigerate. Specimen may be stored at a maximum of 35°C for three days and then at 2°C to 8°C for seven days. Specimen may also be stored frozen (below -70°C).
Quantity not sufficient; inappropriate specimen source; specimen from a pediatric patient; specimen received after leaking out of transport container into the specimen bag; inappropriate or expired specimen transport device; unlabeled specimen container or container with a single patient identifier; name discrepancy between the specimen container label and the computer or the requisition; specimen received after prolonged delay (more than seven days unless frozen); specimen in a paraffin block; specimen in a fixative.
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7018 South Utica Avenue
Tulsa, Oklahoma 74136