Low-Density Lipoprotein Cholesterol (Direct)
Test ID:
703018
CPT code:
83721
Synonyms:
Direct LDL
Direct LDLC
LDL Cholesterol, Direct
LDLC, Direct
Clinical Use:
For the direct determination of LDL cholesterol in nonfasting patients or in patients whose fasting triglycerides are >400 mg/dL, where the estimation of LDL by calculation may not be possible or may lead to inaccuracies. LDL cholesterol measurement, in conjunction with other lipid measurements, has been shown to be useful in assessing the risk of coronary heart disease (CHD). The National Cholesterol Education Program (NCEP)1 has stated that LDL cholesterol should be the “key index” in determination of CHD risk. Laboratory estimation of LDL cholesterol is most commonly determined by the use of formulas, such as the Friedewald formula.2 Use of this formula is limited to fasting samples with triglycerides <400 mg/dL. Triglyceride values between 250−400 mg/dL may also be associated with errors in LDL cholesterol estimation by calculation which, in turn, can lead to misclassification of the patient in regard to the NCEP guidelines.
Specimen Type:
Serum (preferred) or plasma
Requested Volume:
0.5 mL
Minimum Volume:
0.4 mL
Container Type:
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Patient Preparation:
Patients are not required to fast prior to blood collection. Nonfasting and fasting samples can be used. Nonfasting results are slightly lower than fasting results.
Collection:
Separate serum or plasma from cells within 45 minutes of collection.
Storage Instructions:
Room temperature
Stability Requirements:
Temperature |
Period |
---|---|
Room temperature |
14 days |
Refrigerated |
14 days |
Frozen |
14 days |
Freeze/thaw cycles |
Stable x |
Rejection Criteria
Use of anticoagulants containing citrate
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