Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen- activated T lymphocytes.1 IL-2 plays a role in promoting the clonal expansion of antigen-specific cytotoxic and suppressor T cells. In vitro studies suggest that IL-2 may also be produced by dendritic cells and certain lymphoma cell lines. In addition, IL-2 has been shown to mediate multiple immune responses on a variety of cell types. IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation, and cytocidal activity of monocytes; induces the growth of natural killer cells and stimulates cytokine production by these cells, as well as enhancing the cytolytic activity of these cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes.
Red-top tube or gel-barrier tube
Separate serum from cells. Transfer serum to a plastic transport tube.
Stable at room temperature or frozen for 14 days. Freeze/thaw cycles x3.
Gross hemolysis; sample left on cells