Immunofixation (IFE)Serum and Protein Electrophoresis, Serum

Test ID: 


CPT code:

82784(x3) (IgG, IgA, IgM)
84165 (protein electrophoretic fractionation and quantitation)
86334 (immunofixation electrophoresis)


Globulins, Serum

IEP, Serum

IFE, Serum and Protein Electrophoresis, Serum

Immunoelectrophoresis, Serum

Protein IEP

Clinical Use:

Detect and identify monoclonal immunoglobulin gammopathies

Test Information:

Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states.

Specimen Type:


Requested Volume: 

5 mL

Minimum Volume: 

2 mL

Container Type: 

Red-top tube or gel-barrier tube

Patient Preparation: 

Patient should be fasting.


Separate serum from cells.

Storage Instructions:

Separate serum from cells.

Stability Requirements:



Room temperature

3 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Rejection Criteria

Plasma sample

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