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Immunofixation (IFE), Serum, Protein Electrophoresis (PE), Serum, and Quantitative Free κ and λ Light Chains (FLC) Plus Ratio

Test ID: 


CPT code:

82784(3); 83883(x2); 84155; 84165; 86334


Free Light Chains, Quantitative, Serum


Globulins, Serum

IEP, Serum

IFE, Serum and Protein Electrophoresis, Serum

Immunoelectrophoresis for Myeloma Proteins

Kappa Free Light Chains, Quantitative, Serum

Kappa:Lambda Free Light Chains Ratio, Quantitative, Serum

Lambda Free Light Chains, Quantitative, Serum

Light Chains, Free κ and Free λ, Quantitative, Serum

Protein IEP

Serum IFE

Clinical Use:

Detect and identify monoclonal gammopathy (M protein) found on serum protein electrophoresis; diagnose myeloma, macroglobulinemia of Waldenström; evaluate amyloidosis; evaluate lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general; diagnose and characterize immune deficient and dysgammaglobulinemic states

Test Information:

The NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) recommend the use of serum free light chain assays in the initial diagnostic work-up of multiple myeloma and related disorders.
“Use of free light chain (FLC) assay along with SPEP and SIFE yields high sensitivity while screening for MM and related plasma cell disorders. Therefore, this assay is now included as a part of the initial diagnostic work-up in the NCCN Multiple Myeloma Guidelines.”
If screening for plasma cell dyscrasias, the International Myeloma Working Group recommends a screening panel consisting of serum free light chain assay, serum protein electrophoresis (SPEP), and serum immunofixation (IFE) to maximize sensitivity. (A 24-hour urine IFE can be added if AL amyloidosis is suspected.)

Specimen Type:


Requested Volume: 

5.5 mL

Minimum Volume: 

2.2 mL

Container Type: 

Red-top tube or gel-barrier tube

Patient Preparation: 

Patient should be fasting.


Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.

Storage Instructions:

IFE: Refrigerated. PE and FLC: Room temperature

Stability Requirements:



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Rejection Criteria

Plasma sample

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