Test ID: 


CPT code:



HLA B27 Disease Association
Ankylosing Spondylitis
B27 Disease Association
Disease Association Testing

Clinical Use:

Evaluate spondyloarthritis and other disorders associated with HLA-B27

Test Information:

Regarding whole blood specimens, an adequate test requires the presence of intact lymphocytes at the time of testing. Even taking appropriate precautions, an occasional specimen will not be satisfactory for testing. In such cases, fresh blood should be drawn for retesting.
HLA-B27 is strongly associated with ankylosing spondylitis (Marie-Strumpell disease). HLA-B27 shares homology with a Klebsiella protein and may imply a bacterial pathogenesis to ankylosing spondylitis. A patient with consistent clinical and radiographic findings who is B27-positive has a greater chance of having or developing ankylosing spondylitis than a negative patient. The antigen is not causative, however, and 10% of normal subjects are B27-positive. This test should not be considered a screening procedure for ankylosing spondylitis. The antigen is less strongly associated with Reiter syndrome and other arthritides than with ankylosing spondylitis. It has been linked with congenital deficiency of C4 and C2, and with adrenal hyperplasia.

Specimen Type:

Whole blood

Requested Volume: 

Adult tube

Minimum Volume: 

2 mL

Container Type: 

Lavender-top (EDTA) tube

Storage Instructions:

Maintain blood specimen at room temperature.

Rejection Criteria

Hemolysis; clotted specimen; tube other than lavender-top (EDTA) tube

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MCI Diagnostic

Providing top patient care with fast results. 

7018 South Utica Avenue

Tulsa, Oklahoma 74136

Hours of Operation

Mon – Sat: 7AM-11PM

Sun: 7AM-3PM

Government Contract

Mon – Sat: 7AM-11PM

Sun: 7AM-3PM


Mon – Sat: 7AM-11PM

Sun: 7AM-3PM

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