Hemoglobin (Hgb) A1c

Test ID: 

702977

CPT code:

83036

Synonyms:

Glycated Hemoglobin
Hgb A1c

Clinical Use:

Hemoglobin A1c values are used to assess glucose control in diabetes, and in 2010 the American Diabetes Associations affirmed the decision of an international expert committee recommendation to use the A1c test to diagnose diabetes with a threshold ≥6.5%. Point-of-care A1c assays are not sufficiently accurate at this time to use for diagnostic purposes.

Test Information:

Any cause of shortened erythrocyte survival will reduce exposure of erythrocytes to glucose with a consequent decrease in Hb A1c (%). Causes of shortened erythrocyte lifetime might be hemolytic anemia or other hemolytic diseases, homozygous sickle cell trait, pregnancy, or recent significant or chronic blood loss. Glycated Hb F (fetal hemoglobin) is not detected as it does not contain the glycated β chain that characterizes Hb A1c. Specimens containing high amounts of Hb F (>10%) may result in lower than expected Hb A1c.
Factors such as duration of diabetes, adherence to therapy, and age of patient should also be considered in assessing the degree of blood glucose control.

Specimen Type:

Whole blood

Requested Volume: 

4 mL

Minimum Volume: 

Minimum: Pediatric EDTA whole blood tubes may be used. Please place original labeled capillary tube in a labeled transport tube for shipment to the laboratory.

Container Type: 

Lavender-top (EDTA) tube, green-top (lithium heparin) tube, or gray-top (sodium fluoride) tube

Collection:

The usual precautions in the collection of venipuncture samples should be observed. The sample must be free of clots. Samples with any hematocrit disorders can lead to erroneous results. Send the entire tube to the laboratory.

Storage Instructions:

Maintain specimen at room temperature.

Stability Requirements:

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Rejection Criteria

Clotted specimen; gross hemolysis

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