Helicobacter pylori Culture
Campylobacter From Stomach
H pylori Culture
Culture, Helicobacter pylori
Establish the presence and possible etiologic role of Helicobacter pylori in cases of chronic gastric ulcer, gastritis, duodenal ulcer, dyspepsia, etc
For optimal recovery of Helicobacter pylori, it is strongly recommended that culture be performed within three hours of specimen collection. Helicobacter pylori has been implicated as a factor associated with chronic gastritis. The clinical significance of the organism in regard to gastric or duodenal ulcers, dyspepsia, and gastric carcinoma remains unclear.1 Other factors, such as pepsin, nonsteroidal anti-inflammatory agents (NSAIDs), aspirin, ischemia, stress, alcohol, and bile salts as well as H pylori, all may have a role in altering the material mucosal barrier. The relative etiologic role of H pylori remains a subject of speculation. H pylori is hypothesized to secrete products capable of degrading gastric mucin. The organism may be seen in Gram stains on imprints from tissue biopsies. In one study, imprint Gram stains performed by imprint technique on biopsies from both the antrum and fundus yielded positives in 100% of 32 culture-positive cases.
Anaerobic transport, aerobic/anaerobic bacterial swab transport system, CLO vial, urea agar medium, C&S transport (Cary Blair/orange Para-Pak)
Maintain specimen at room temperature.
Inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 72 hours)