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Gram Stain

Test ID: 


CPT code:


Clinical Use:

Determine the presence of microörganisms and to evaluate the type of specimen by type of cells seen (eg, PMN, epithelial)

Specimen Type:

Material from infected area

Requested Volume: 

Smear (air-dried) made at bedside or immediately after collection is preferred or one made in laboratory from swab or clinical material.

Container Type: 

Clean glass slides, swab in transport or clinical material in sterile container


Carefully select material from infected area with a sterile swab. Gently roll swab onto a clean glass slide to make a thin smear. Air dry the slide. Do not fix.

Storage Instructions:

Maintain specimen at room temperature.

Rejection Criteria

Inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 48 hours); stool or fecal source (see White Blood Cells (WBC).

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