G-6-PD, Quant, Blood and Hgb

Test ID: 

702944

CPT code:

82955, 85018

Synonyms:

G6PD, Quantitative, Blood and Hgb
G6PDH

Clinical Use:

Evaluate glucose 6-phosphate dehydrogenase (G6PD) deficiency. G6PD deficiency, an X-linked disorder, is the most common enzymatic disorder of red blood cells in humans, affecting more than 400 million people worldwide. The clinical expression of G6PD variants encompasses a spectrum of hemolytic syndromes. Affected patients are most often asymptomatic, but many patients have episodic anemia, while a few have chronic hemolysis.

Test Information:

G6PD hemolysis is associated with formation of Heinz bodies in peripheral red blood cells. It is the older erythrocytes that are most G6PD-deficient in affected individuals. These cells are first eliminated in a hemolytic crisis. The younger cells and reticulocytes contain more G6PD. For these reasons, after a hemolytic crisis, when only younger erythrocytes and reticulocytes are present, the G6PD values may be spuriously normal.

Specimen Type:

Whole blood

Requested Volume: 

8 mL (4 mL in each of two tubes)

Minimum Volume: 

Hemoglobin: two 500-μL lavender-top Microtainer™ tubes filled to at least 50% of tube capacity (Note: If any other size lavender tube is used, the tube must be filled to at least 50% capacity of tube fill volume. Insufficient volume may limit the extent of procedures performed.); and G6PD: one lavender-top (EDTA) tube, green-top (heparin) tube, or yellow-top (ACD) tube (0.1 mL) whole blood.

Container Type: 

Two lavender-top (EDTA) tubes or one green-top (heparin) tube and one lavender-top (EDTA) tube or one yellow-top (ACD) tube and one lavender-top (EDTA) tube

Storage Instructions:

Hemoglobin: Stable refrigerated for 72 hours. G6PD: Stable refrigerated for seven days.

Rejection Criteria

Hemoglobin: Hemolysis; tube not filled with minimum fill volume; specimen drawn in any anticoagulant other than EDTA; specimens diluted or contaminated with IV fluid; clotted specimen; improper labeling; transfer tubes with whole blood; lavender-top (EDTA) tubes received with plasma removed; samples more than 72 hours old. G6PD: Frozen specimen.

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