Fungus Culture With Stain
Aid in the establishment of the diagnosis of fungal infection
Aspirate, biopsy, body fluid, bronchoalveolar lavage (BAL), nails, hair, skin, sinus, sputum, stool, urine, or genital
2 mL or 1 cc tissue; whole nails or scrapings, hairs, skin scrapings; 50 mL body fluid; 5 mL aspirates
Sterile container with fluid or tissue; bacterial swab transports (two swabs: one for culture, one for stain)
Refrigerate respiratory specimens that are contaminated with oropharyngeal flora; other specimens should be at room temperature.
Fungal stain (KOH/Calcofluor) cannot be performed on blood; unlabeled specimen or name discrepancy between specimen and request label; specimen submitted in syringe with needle attached; inappropriate transport device; swab without evidence of specimen present; specimen received after prolonged transport (usually more than 72 hours); specimens received after leaking transport container into specimen bag. Trach-suction devices will often leak if the cap with tubing is not removed and replaced by a solid cap. This may need to be done by personnel collecting the specimen as the solid cap is usually in with the device. If there is not solid cap, the specimen should be transferred to a leakproof sterile cup with metal cap.
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