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Coombs’, Direct

Test ID: 


CPT code:




Direct Antihuman Globulin TesT

Direct Coombs’

Clinical Use:

Detect in vivo coating of IgG and/or complement components on RBCs

Test Information:

Positive direct antiglobulin tests are associated with a variety of conditions which include hemolytic transfusion reaction, autoimmune hemolytic anemia, hemolytic disease of the fetus/newborn, and may be secondary to certain pathological conditions or drug therapy. Note: The presence of a positive direct antiglobulin test does not necessarily correlate with a pathological condition.

Specimen Type:

Whole blood

Requested Volume: 

4 mL

Minimum Volume: 

0.5 mL

Container Type: 

Lavender-top (EDTA) tube or yellow-top (ACD) tube

Storage Instructions:

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.

Rejection Criteria

Gross hemolysis; bacterial or other contamination; specimen more than five days old

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