Chromosome, Leukemia/Lymphoma

Test ID: 

703221

CPT code:

88237; 88264; 88280 (x2)

Synonyms:

Chromosome Analysis, Leukemia/Lymphoma

Chromosome Analysis, Hematologic Disorders

Lymphoma

Clinical Use:

Karyotyping, physical localization of copy number changes, and mapping of breakpoints involved in translocations.

Specimen Type:

Bone marrow, blood (>4% blasts required − see Collection), lymph node, spleen, bone core, or effusion

Requested Volume: 

2 mL bone marrow, 3 mL peripheral blood, or 0.5 to 1 cm³ tissue biopsy

Container Type: 

Bone marrow or peripheral blood in pediatric green-top (heparin) tube; lymph node, spleen, bone core, CSF, or effusion in lymph node transport bottle

Collection:

The requirement of >4% blasts for blood does not apply to cases of CLL, hairy cell leukemia, and some cases of small-cell lymphocytic leukemia (SCLL) or lymphoma, which can be β-mitogen stimulated. Submit bone marrow or peripheral blood at room temperature using Leukemia/Lymphoma Specimen Transport Kit. Submit lymph node, spleen, or bone core at room temperature using Lymph Node Transport Kit. Effusions may be sent in small sterile containers. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the test request form.

Storage Instructions:

Maintain specimen at room temperature.

Rejection Criteria

Hemolysis; gross contamination; non-neoplastic cells; clotted specimen; frozen specimen; fixed specimen; improper or too much anticoagulant

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