88237; 88264; 88280 (x2)
Chromosome Analysis, Leukemia/Lymphoma
Chromosome Analysis, Hematologic Disorders
Karyotyping, physical localization of copy number changes, and mapping of breakpoints involved in translocations.
Bone marrow, blood (>4% blasts required − see Collection), lymph node, spleen, bone core, or effusion
2 mL bone marrow, 3 mL peripheral blood, or 0.5 to 1 cm³ tissue biopsy
Bone marrow or peripheral blood in pediatric green-top (heparin) tube; lymph node, spleen, bone core, CSF, or effusion in lymph node transport bottle
The requirement of >4% blasts for blood does not apply to cases of CLL, hairy cell leukemia, and some cases of small-cell lymphocytic leukemia (SCLL) or lymphoma, which can be β-mitogen stimulated. Submit bone marrow or peripheral blood at room temperature using Leukemia/Lymphoma Specimen Transport Kit. Submit lymph node, spleen, or bone core at room temperature using Lymph Node Transport Kit. Effusions may be sent in small sterile containers. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the test request form.
Maintain specimen at room temperature.
Hemolysis; gross contamination; non-neoplastic cells; clotted specimen; frozen specimen; fixed specimen; improper or too much anticoagulant
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