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Chromosome, AFP/AChE/HbF, Amn

Test ID: 


CPT code:

82106, 82013


Chromosome Analysis, AFP, AChE, Amniotic Fluid With Reflex to Fetal Hemoglobin (Hb F)

Clinical Use:

Determine fetal karyotype; prenatal diagnosis of Down syndrome or other chromosomal abnormalities; analysis of midtrimester amniotic fluid for detection of open neural tube and ventral wall defects

Specimen Type:

Amniotic fluid

Requested Volume: 

20 to 30 mL

Minimum Volume: 

10 mL

Container Type: 

Sterile plastic conical tube; do not use urine containers or tubes with rubber stoppers. Rubber is toxic to amniocytes.


Discard first 2 mL of fluid aspirated to avoid maternal cell contamination. Specimen is collected in a 20 mL sterile syringe and transferred aseptically to sterile tubes. The test request form is completed and accompanies specimen and miscellaneous slip to the laboratory.

Storage Instructions:

Maintain specimen at room temperature. Do not freeze or refrigerate.

Stability Requirements:



Room temperature

1 month

Rejection Criteria

Specimen found not to be amniotic fluid; gross contamination with red cells; frozen specimen; nonsterile container. Do not use urine containers or tubes with rubber stoppers; rubber is toxic to amniocytes.

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