Cholinesterase, Plasma and RBC
True cholinesterase (RBC and plasma) activity is decreased in individuals with exposure to organophosphorous insecticides. True cholinesterase, found in erythrocytes and nerve tissue, is responsible for inactivating acetylcholinesterase at nerve endings. With decreased enzyme activity, patients may display a range of nervous system dysfunction. Analysis of RBC and serum or plasma activity is useful in monitoring exposure and recovery.
Plasma and whole blood
2 mL plasma and 5 mL whole blood
0.1 mL plasma and 1 mL whole blood
Lavender-top (EDTA) tube and transport tube marked “plasma”
Separate plasma from cells for one lavender-top tube, only. Place in transport tube and mark “plasma.” Do not separate other EDTA tube.
Room temperature. .
Whole blood (RBC): Stable at room temperature or refrigerated for 14 days and unstable frozen. Plasma:Stable at room temperature, refrigerated, or frozen for 14 days; freeze/thaw cycles: stable x3
Plasma and whole blood samples not sent; anticoagulants other than EDTA (RBC only); frozen whole blood received; hemolyzed plasma or whole blood
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