Bordetella pertussis, Nasopharyngeal Culture
Bordetella pertussis Culture
Culture, Bordetella pertussis, Nasopharyngeal
Whooping Cough Culture
Isolate and identify B pertussis and B parapertussis; establish diagnosis of whooping cough
Additional Test Information:
Nucleic Acid Amplification (NAA/PCR) procedures provide more rapid results and have been increasingly used in the diagnosis of B pertussis infection. Studies have shown that the best yield is obtained when PCR and culture are used to diagnose this infection.
Nasopharyngeal (NP) swab
Flexible swab and special charcoal-containing Bordetella transport media (available from laboratory).
Patient must not be on antimicrobial therapy.
Guide the flexible swab into the contour of the nares and into the nasopharynx. Pass the swab gently through the nose. Leave swab in place near septum and floor of nose for 15 to 30 seconds. Rotate and remove. Place into special transport media available from the laboratory.
If possible, preincubate at 37°C; then use refrigerated temperature (2°C to 8°C) for transport.
Unlabeled specimen or name discrepancy between specimen and request label; prolonged delay in transport (usually more than 72 hours); inappropriate specimen transport device (eg, noncharcoal-containing transport medium)
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