Antithrombin Activity
Test ID:
706584
CPT code:
85300
Synonyms:
- Antithrombin III Activity
- Antithrombin III, Functional
- AT3 Activity
- Factor Xa Inhibitor
- Heparin Cofactor Activity
- Serine Protease Inhibitor
Clinical Use:
Confirmation and characterization of acquired or congenital AT deficiency
Specimen Type:
Plasma, frozen
Requested Volume:
2 mL
Minimum Volume:
1 mL
Container Type:
Blue-top (sodium citrate) tube
Patient Preparation:
Do not draw from an arm with a heparin lock or heparinized catheter. Patients ideally should not be on unfractionated heparin or direct Xa inhibitor anticoagulant therapy.
Collection:
Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples unless the sample is collected using a winged (butterfly) collection system. With a winged blood collection set a discard tube should be drawn first to account for the dead space of the tubing and prevent under-filling of the evacuated tube.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternative anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.
Storage Instructions:
Freeze.
Rejection Criteria
Severe hemolysis; improper labeling; clotted specimen; specimen diluted with IV fluids; samples thawed in transit; improper sample type; sample out of stability
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