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Androsterone, Serum, LCMS

Test ID: 


CPT code:


Clinical Use:

Androsterone is a major metabolite of testosterone and androstenedione along with the enantiomer etiocholanolone. Androsterone is also part of the “backdoor pathway” from allopregnanolone to dihydrotestosterone, which has importance in certain disease states. Androsterone is one of a few steroids with neutral activity that interact with γ-aminobutyric acid (GABA) receptors

Specimen Type:

Serum (preferred) or EDTA plasma, frozen

Requested Volume: 

1 mL

Minimum Volume: 

0.4 mL (Note: This volume does not allow for repeat testing

Container Type: 

Red-top tube (Do not use gel-barrier tube.) or lavender-top (EDTA) tube

Patient Preparation: 

Fasting specimen is preferred. Patient should avoid any hormonal medications for 2 days.


Centrifuge and separate serum or plasma immediately. Freeze. Send serum or plasma in a plastic transport tube.

Storage Instructions:

Freeze. Stable at room temperature for nine hours. Stable refrigerated and frozen for seven days. Freeze/thaw cycles stable x6.

Rejection Criteria

Sample received thawed; gross hemolysis

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