Anaerobic Culture

Test ID: 

705691

CPT code:

87075

Synonyms:

Anaerobic Culture, Abscess/Anaerobic Culture, Wound/Culture, Anaerobic/Wound Anaerobic Culture

Clinical Use:

Isolate and identify anaerobic pathogenic organisms; determine susceptibility of isolates (extra charge). When actinomycetes are suspected a specific request must be made

Specimen Type:

Pus, tissue, or other material properly obtained from an abscess, biopsy, aspirate, drainage, exudate, lesion, or wound. To ensure proper growth of organisms place swabs/specimen in anaerobic transporter

Requested Volume: 

1 mL

Minimum Volume: 

0.5 ml this volume does not allow for repeat testing

Container Type: 

Anaerobic transport or aerobic/anaerobic bacterial swab transport containing gel medium

Patient Prep:

Sterile preparation of the aspiration site is imperative.

Collection:

Some anaerobes will be killed by contact with molecular oxygen for only a few seconds. Overlying and adjacent areas must be carefully disinfected to eliminate contamination with indigenous flora. Ideally, pus or other fluid obtained by needle aspiration through intact skin or mucosal surface that has been cleaned with antiseptic should be collected.

Storage:

Specimens for anaerobic culture should be maintained at room temperature. Under these conditions, anaerobes will survive 24 to 72 hours when properly collected in the anaerobic transport tube. Storage of specimens in the ESwab™ transport at room temperature for greater than 48 hours may result in diminished recovery of certain anaerobic species.

Rejection Criteria

Unlabeled specimen or name discrepancy between specimen and test request label; specimen not received in appropriate anaerobic transport tube; expired transport; swab not in gel transport medium or ESwab™ transport; swab not stored in oxygen-free atmosphere; specimen refrigerated. Note: Refrigeration inhibits viability of certain anaerobic organisms. If an unacceptable specimen is received, the client will be notified and another specimen will be requested before disposal of the original specimen. Specimens from sites that have anaerobic bacteria as indigenous flora will not routinely be cultured anaerobically (eg, throat, feces, colostomy stoma, rectal swabs, bronchial washes, cervical-vaginal mucosal swabs, sputa, skin and superficial wounds, voided or catheterized urine, ulcer surfaces, drainages onto contaminated surfaces)

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