Anaerobic and Aerobic Culture and Gram Stain
87070, 87075, 87205
Anaerobic Culture, Abscess/Anaerobic Culture, Wound/Culture, Anaerobic/Wound Anaerobic Culture
Isolate and identify anaerobic pathogenic organisms; determine susceptibility of isolates (extra charge). When actinomycetes are suspected a specific request must be made
Pus, tissue, or other material properly obtained from an abscess, biopsy, aspirate, drainage, exudate, lesion, or wound. To ensure proper growth of organisms place swabs/specimen in anaerobic transporter. Do not refrigerate.
Swab(s) in aerobic/anaerobic swab transport or 0.5 mL pus, other fluid, or tissue from aspirated site in anaerobic transporter and one thin smear
Aerobic/anaerobic bacterial swab transport containing gel preservative or anaerobic transporter and one prepared smear in slide carrier
Sterile preparation of the aspiration site is imperative.
Some anaerobes will be killed by contact with molecular oxygen for only a few seconds. Overlying and adjacent areas must be carefully disinfected to eliminate contamination with indigenous flora. Ideally, pus or other fluid obtained by needle aspiration through intact skin or mucosal surface that has been cleaned with antiseptic should be collected
Specimens for anaerobic culture should be maintained at room temperature. Under these conditions, aerobes and anaerobes will survive 24 to 72 hours when properly collected in the anaerobic transport tube
Unlabeled specimen or name discrepancy between specimen and test request label; specimen not received in appropriate anaerobic transport tube; swab not in gel transport medium; ESwab™; swab not stored in oxygen-free atmosphere; specimen refrigerated; specimen received after prolonged delay in transport (usually more than 72 hours).
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