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Acetylcholinesterase (AChE), Amniotic Fluid With Reflex

Test ID: 


CPT code:



•Amniotic Fluid With Reflex to Hb F

Clinical Use:

Analysis of midtrimester amniotic fluid for detection of open neural tube defects and ventral wall defects.

Additional Test Information:

The patient’s gestational age must be at least 13 weeks for accurate AChE detection. Optimal gestational age is 14 to 18 weeks. Include the gestational age by ultrasound and/or last menstrual period (LMP) on the request form.

Specimen Type:

Amniotic fluid

Requested Volume: 

3 mL

Container Type: 

Sterile plastic conical tube; do not use urine containers or tubes with rubber stoppers. Rubber is toxic to amniocyte

Patient Preparation: 

The patient should have undergone ultrasound studies to verify fetal viability, detect multiple gestation, confirm gestational age, localize placenta, and detect fetal and uterine pathology.


Avoid contamination of amniotic fluid with maternal or fetal blood. As little as one drop of fetal blood can cause false-positive results during assay of amniotic fluid. Amniotic fluid should be collected by the attending physician.

Storage Instructions:

Ship and maintain specimen at room temperature.

Rejection Criteria

Specimen found not to be amniotic fluid; gross contamination of amniotic fluid with maternal or fetal blood; quantity not sufficient for analysis.

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